Sign in →

Test Code SECOS Secobarbital, Serum

Reporting Name

Secobarbital, S

Useful For

Monitoring secobarbital therapy

Method Name

Gas Chromatography-Mass Spectrometry (GC-MS)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum Red


Specimen Required


Container/Tube: Red top

Specimen Volume: 1.5 mL

Collection Instructions: Spin down within 2 hours of draw.


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

Serum gel tube

Reference Values

Therapeutic concentration: 1.0-2.0 mcg/mL

Toxic concentration: >5.0 mcg/mL

Day(s) and Time(s) Performed

Monday

CPT Code Information

80345

G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SECOS Secobarbital, S 4028-7

 

Result ID Test Result Name Result LOINC Value
8243 Secobarbital, S 4028-7

Analytic Time

1 day

Cautions

The concentration at which toxicity occurs varies and results should be interpreted in light of the clinical situation.

Method Description

Barbiturates are extracted from serum using solid-phase extraction techniques. The serum is buffered and eluted with organic solvent. The organic phase is dried, reconstituted, and analysis is performed by gas chromatography-mass spectrometry, using selected ion monitoring. The assay utilizes deuterated barbiturates as internal standards.(Unpublished Mayo method)

Interpretation

Secobarbital concentrations above 5 mcg/mL have been associated with toxicity.

Specimen Retention Time

2 weeks

Clinical Information

Secobarbital is a short-acting barbiturate with hypnotic properties used as a preanesthetic agent and in the short-term treatment of insomnia.(1,2)

 

Secobarbital is administered orally. The duration of its hypnotic effect is about 3 to 4 hours. The drug distributes throughout the body, with a volume of distribution (Vd) of 1.6 to 1.9 L/kg, and about 46% to 70% of a dose is bound to plasma proteins. Metabolism takes place in the liver primarily via hepatic microsomal enzymes. Secobarbital's half-life is about 15 to 40 hours (mean: 28 hours).(2,3)

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Forms

If not ordering electronically, complete, print, and send a Neurology Specialty Testing Client Test Request (T732) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/neurology-request-form.pdf)