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Test Code LAB2705 T- and B-Cell Markers, Whole Blood

Additional Codes

Test Name in EPIC EPIC Test Code Mnemonic



Flow Cytometry/Immunostaining

Includes percent cells and absolute counts for CD3 (T-cells), CD4 (helper cells), CD8 (suppressor cells), CD19 (B-cells), CD56+/CD3- (natural killer cells), and helper/suppressor ratio.

Specimen Requirements

Specimen must be sent to SMDC Main Laboratory the day of collection or ASAP thereafter. Testing must be completed within 48 hours of draw.


Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 2 mL

Specimen Minimum Volume: 0.5 mL

Additional Information: Specimen cannot be frozen.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO

Day(s) Test Set Up

Monday through Sunday (day shift only)

Reference Values


Absolute counts

CD3: 537-2,939 cells/µL

CD4: 321-2,124 cells/µL

CD8: 46-1,346 cells/µL

CD19: 14-816 cells/µL

CD56+/CD3-: 13-441 cells/µL

Percent cells

CD3: 56-93%

CD4: 29-76%

CD8: 5-49%

CD19: 1-37%

CD56+/CD3-: 2-26%

Helper/suppressor ratio: ≥1.0

Test Classification and CPT Coding

86355-Absolute B cell count

86359-T-cells, total count

86360-Absolute CD4 and CD8 count, including ratio

86357-Absolute natural killer cell count

Performing Laboratory

Special Hematology-

SMDC Clinical Lab