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Test Code CIFST Cutaneous Immunofluorescence Antibodies Titer, IgG, Serum


Specimen Required


Only orderable as a reflex. For more information see CIFS / Cutaneous Immunofluorescence Antibodies, IgG and IgG4, Serum.

 

Collection Container/Tube:

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Confirmation of positive IgG anti-cell surface (CS) and anti-basement membrane zone (BMZ) antibodies.

Method Name

Only orderable as a reflex. For more information see CIFS / Cutaneous Immunofluorescence Antibodies, IgG and IgG4, Serum.

 

Indirect Immunofluorescence Assay (IFA)

Reporting Name

CIFS Titer, IgG, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Clinical Information

Immunoglobulin G (IgG) anti-basement membrane zone (BMZ) antibodies are produced by patients with pemphigoid. In most patients with bullous pemphigoid, serum contains IgG anti-BMZ antibodies, while in cicatricial pemphigoid circulating IgG anti-BMZ antibodies are found in a minority of cases.

 

Circulating IgG anti-BMZ antibodies are also detected in patients with epidermolysis bullosa acquisita and bullous eruption of lupus erythematosus.

 

Immunoglobulin G anti-cell surface (CS) antibodies are produced by patients with pemphigus. The titer of IgG anti-CS antibodies generally correlates with disease activity of pemphigus.

Reference Values

Only orderable as a reflex. For more information see CIFS / Cutaneous Immunofluorescence Antibodies, IgG and IgG4, Serum.

 

Negative in normal individuals

Interpretation

Indirect immunofluorescence (IF) testing may be diagnostic when histologic or direct IF studies are only suggestive, nonspecific, or negative.

 

Anti-cell surface antibodies correlate with a diagnosis of pemphigus.

 

Anti-basement membrane zone antibodies correlate with a diagnosis of bullous pemphigoid, cicatricial pemphigoid, epidermolysis bullosa acquisita, or bullous eruption of lupus erythematosus.

Cautions

Results should be interpreted in conjunction with clinical information, histologic pattern, and results of direct immunofluorescence (IF) study. In particular, the finding of low titer (≤1:80) anti-cell surface antibodies should not be used alone (ie, without histologic or direct IF support) to confirm a diagnosis of pemphigus.

Method Description

Frozen sections of primate esophagus are overlaid with dilutions of patient's serum, incubated, covered with fluorescein-conjugated IgG antiserum, and interpreted with a fluorescence microscope.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

2 to 7 days

Specimen Retention Time

30 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88350

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CIFST CIFS Titer, IgG, S 104835-4

 

Result ID Test Result Name Result LOINC Value
622323 Cell Surface Ab Titer, IgG 104831-3
622324 Basement Membrane Titer, IgG 104836-2
622325 Other 48767-8