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HelpTest Code CYTH1 T-Helper Cell Type 1 Cytokine Panel, Plasma
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Immediately after specimen collection, place the tube on wet ice.
2. Within 2 hours of collection, centrifuge at 4° C, 1500 x g for 10 minutes and aliquot plasma into a plastic vial
3. Freeze immediately.
Additional Information:
1. Plasma for this test cannot be shared with other tests. Submit specimen for this test in its own vial.
2. This test cannot be added on to a previously collected specimen.
Useful For
Assessment of plasma cytokine levels to understand the etiology of autoimmune and proinflammatory conditions when used in conjunction with clinical information and other laboratory testing
Method Name
Bead-Based Multiplex Immunoassay
Reporting Name
Th1 Cytokine Panel, PSpecimen Type
Plasma EDTASpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-treated specimen | Reject |
Clinical Information
Cytokines are important mediators of cell-to-cell communication within the innate and adaptive immune systems. The expression of most cytokines is highly regulated, and generally occurs in response to foreign or self-antigenic stimulation. The functions of cytokines are extremely varied, with many cytokines also displaying pleiotropic effects, depending on their cellular target. As a group, cytokines and their receptors represent a highly complex and critical regulator of a normal immune response.
Reference Values
TNF:
<18 years: Not established
≥18 years: <10.0 pg/mL
IFN-gamma:
<18 years: Not established
≥18 years: <60.0 pg/mL
IL-2:
<18 years: Not established
≥18 years: <60.0 pg/mL
IL-2 receptor alpha soluble:
<18 years: Not established
≥18 years: ≤959 pg/mL
IL-12p70:
<18 years: Not established
≥18 years: <200 pg/mL
Interpretation
Elevated cytokine concentrations could be consistent with the presence of infection or other inflammatory process.
Cautions
Results from cytokine testing should not be used to establish or exclude a specific diagnosis.
Cytokine testing should only be used in conjunction with clinical information and other laboratory testing as part of a patient's overall assessment.
Normal concentrations of cytokines do not exclude the possibility of infection or other inflammatory condition.
Cytokine concentrations could be affected by immunomodulatory agents.
Method Description
Testing for plasma cytokine levels is accomplished using a laboratory-developed immunoassay.(Unpublished Mayo method)
Day(s) Performed
Thursday
Report Available
2 to 8 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
83520 x5
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CYTH1 | Th1 Cytokine Panel, P | 82335-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 622037 | TNF | 3074-2 |
| 622038 | IFN-gamma | 27415-9 |
| 622039 | IL-2 | 33939-0 |
| 622040 | IL-2 receptor alpha soluble | 76039-7 |
| 622041 | IL-12p70 | 41760-0 |