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HelpTest Code JCV JC Virus Detection by In Situ Hybridization
Shipping Instructions
Attach the green pathology address label included in the kit to the outside of the transport container.
Necessary Information
A pathology/diagnostic report and a brief history are required.
Specimen Required
Specimen Type: Formalin-fixed, paraffin-embedded tissue block
Supplies: Pathology Packaging Kit (T554)
Specimen Volume: Entire block
Specimen Type: Slides
Slides: 4 Unstained glass, positively-charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue
Useful For
Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy
Method Name
In Situ Hybridization (ISH)
Reporting Name
JC Virus ISHSpecimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Special | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides |
Reject |
Clinical Information
JC virus is the etiologic agent of progressive multifocal leukoencephalopathy (PML), a rare, demyelinating, fatal disorder of the central nervous system that occurs on a background of immune deficiency. PML is an infrequent complication of a wide variety of conditions, including lymphoproliferative disorders (Hodgkin disease, chronic lymphocytic leukemia), sarcoidosis, tuberculosis, and AIDS.
Interpretation
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.
Cautions
Studies have shown potential cross reactivity between polyoma (BK) and JC virus in situ hybridization (ISH) testing.(1)
Age of a cut paraffin section can affect staining quality. Stability thresholds vary widely among published literature. Best practice is for paraffin sections to be cut within 6 weeks.
Method Description
In situ hybridization on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Report Available
5 to 7 daysSpecimen Retention Time
Until staining is complete.Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88365-Primary
88364-If additional ISH
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| JCV | JC Virus ISH | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 71214 | Interpretation | 50595-8 |
| 71215 | Participated in the Interpretation | No LOINC Needed |
| 71216 | Report electronically signed by | 19139-5 |
| 71218 | Material Received | 81178-6 |
| 71596 | Disclaimer | 62364-5 |
| 72115 | Case Number | 80398-1 |
Forms
If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.