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HelpTest Code LAB2613 Coagulation Factor V Activity Assay, Plasma
Additional Codes
| Test Name in EPIC | EPIC Test Code | Mnemonic |
|---|---|---|
| FACTOR V ASSAY | LAB2613 | FAC5 |
Useful For/Utility
Diagnosing congenital deficiencies (rare) of coagulation factor V. Evaluating acquired deficiencies associated with liver disease, factor V inhibitors, myeloproliferative disorders, and intravascular coagulation and fibrinolysis. Investigation of prolonged prothrombin time or activated partial thromboplastin time.
This test is not used for the diagnosis of factor V Leiden mutation.
Methodology
Electromechanical Clot Detection
Clinical Information
Factor V is a vitamin K-independent protein synthesized in the liver and in other tissues (endothelium, megakaryocytes/platelets). Deficiency of factor V may cause prolonged prothrombin time and activated partial thromboplastin time and may result in a bleeding diathesis. Plasma biological half-life varies from 12 to 36 hours.
Specimen Requirements
Fill Blue top tube to the etched line located just below the cap of the tube.
1. Light-blue top (3.2% sodium citrate); tube must be properly filled
2. Short-draws, over-draws, clotted or hemolyzed specimens may yield incorrect results.
3. Glass vials are not acceptable for processing, transport or storage.
Specimen Transport Temperature
1. Do not spin in refrigerated centrifuge.
2. Double-centrifuged specimens are critical for accurate results - spin initial specimen, remove top 2/3 of plasma, and spin plasma again in polypropylene vial.*
3. Aliquot top 3/4 of respun specimen and place platelet-poor plasma in polypropylene vial.*
4. If there is a delay in transport or testing for > 4 hours, send specimen frozen.
5. Preferred specimen volume: 1 mL plasma
6. Minimum specimen volume: 0.5 mL plasma
* 2 Aliquot labels will auto print at non-performing sites to double spin
Day(s) Test Set Up
Upon request (day shift only)
Performing Laboratory
Coagulation-
SMDC Clinical Lab
Test Classification and CPT Coding
85220
Reference Values
Reference Values
| TEST | AGE | % Activity |
|---|---|---|
|
Factor V Assay |
0 – 2 days |
64 - 103 |
|
|
3 days – 30 days |
92 - 154 |
|
|
31 days – 1 Year |
94 -141 |
|
|
1 Year - 5 Years |
67 - 127 |
|
|
6 Years - 10 Years |
56 - 141 |
|
|
11 Years - 17 Years |
67 - 141 |
|
|
18 Years - 150 Years |
62 - 139 |
Normal, full term infants may have boderline low or mildly decreased levels (greater than or equal to 30-35%) which may remain reach adult levels within 21 days postnatal. Healthy premature infants (30-36 weeks gestation) may have boderline low or mildly decreased levels. (per Mayo Medical Laboratories;4/2014)