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HelpTest Code LAB2613 Coagulation Factor V Activity Assay, Plasma
Additional Codes
| Test Name in EPIC | EPIC Test Code | Mnemonic |
|---|---|---|
| FACTOR V ASSAY | LAB2613 | FAC5 |
Methodology
Electromechanical Clot Detection
Clinical Information
Factor V is a vitamin K-independent protein synthesized in the liver and in other tissues (endothelium, megakaryocytes/platelets). Deficiency of factor V may cause prolonged prothrombin time and activated partial thromboplastin time and may result in a bleeding diathesis. Plasma biological half-life varies from 12 to 36 hours.
Specimen Requirements
Specimen Type: Platelet-poor plasma
Container/Tube:
Fill Blue top tube to the etched line located just below the cap of the tube.
Preferred: Full Light-blue top (3.2% sodium citrate)
Acceptable: Full Sodium Citrate (BD® or Greiner tubes)
Specimen Volume: 1 mL
Specimen Minimum Volume: 0.5 mL
Collection Instructions:
1. Do not spin in refrigerated centrifuge.
2. Unacceptable specimens: Specimens that are short-draws, over-draws, clotted or hemolyzed may yield incorrect results.
3. If sending from a site spin down, remove top 2/3 of plasma, and spin plasma again in a polypropylene (cloudy plastic) vial.
4. Aliquot top 3/4 and place platelet-poor plasma in a polypropylene (cloudy plastic) vial.
5. Glass vials are not acceptable for processing/transport/storage.
Additional Information:
1. Double-centrifuged specimens are critical for accurate results as platelet contamination may cause spurious results.
2. If there is a delay in transport of > 4 hours, send specimen frozen.
Specimen Transport Temperature
Refrigerate* 8 hours/Frozen 15 days at -20° C or 1 month at -80° C OK/Ambient 4 hours at 20° ± 5° C OK
*Note: If the same plasma is also to be used for testing of Factor VII, do not store at 2-8° C.
Day(s) Test Set Up
Upon request (day shift only)
Performing Laboratory
Coagulation-
SMDC Clinical Lab
Test Classification and CPT Coding
85220
Reference Values
Reference Values
| TEST | AGE | % Activity |
|---|---|---|
|
Factor V Assay |
0 – 2 days |
64 - 103 |
|
|
3 days – 30 days |
92 - 154 |
|
|
31 days – 1 Year |
94 -141 |
|
|
1 Year - 5 Years |
67 - 127 |
|
|
6 Years - 10 Years |
56 - 141 |
|
|
11 Years - 17 Years |
67 - 141 |
|
|
18 Years - 150 Years |
62 - 139 |
Normal, full term infants may have boderline low or mildly decreased levels (greater than or equal to 30-35%) which may remain reach adult levels within 21 days postnatal. Healthy premature infants (30-36 weeks gestation) may have boderline low or mildly decreased levels. (per Mayo Medical Laboratories;4/2014)
Userful For/Utility
Diagnosing congenital deficiencies (rare) of coagulation factor V. Evaluating acquired deficiencies associated with liver disease, factor V inhibitors, myeloproliferative disorders, and intravascular coagulation and fibrinolysis. Investigation of prolonged prothrombin time or activated partial thromboplastin time.
This test is not used for the diagnosis of factor V Leiden mutation.