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HelpTest Code LAB4314 Factor V Leiden Mutation, Whole Blood
Additional Codes
| Test Name in EPIC | EPIC Test Code | Mnemonic |
|---|---|---|
| Factor V Leiden Mutation, Whole Blood | LAB4314 | EH F5DNA |
Useful For/Utility
Aid in the diagnosis of suspected thrombophilia
Performing Laboratory
SMDC Clinical Lab
Specimen Type
EDTA Whole Blood in original collection tube
Specimen Required
4.0 mL EDTA whole blood (Minimum: 0.5 mL EDTA whole blood)
Specimen Stability Information
Do not centrifuge or concentrate the whole blood sample by plasma removal.
Samples should be stored at 2-8C if transport to performing laboratory is longer than 24 hours.
Whole blood is stable up to 15 days when stored at 2-8C.
Whole blood samples may be stored at -20C or -80C for up to 3 months (use of freezer compatible storage vial is recommended).
Refrigerate - 15 days
Method and Method Description
Cepheid GeneXpert System by real-time polymerase chain reaction (PCR)
This test uses the Cepheid Xpert FII & FV assay to detect Factor V Leiden (G1691A) mutations using real-time polymerase chain reaction (PCR) on the Cepheid GeneXpert Instrument System. The primers and probes in the Xpert FII & FV assay determine the genotype of the Factor V gene (at position 1691). Factor V Leiden (G1691A) refers to the G to A transition at nucleotide position 1691 of the Factor V gene, resulting in the substitution of amino acid arginine by glutamine in the Factor V protein, causing resistance to cleavage by Activated Protein C (APC). Factor V Leiden (G1691A) mutations are present in 5% of the general population. The Cepheid Xpert FII & FV assay is FDA-cleared; its performance was verified by Essentia Health-SMDC Clinical Laboratory.
Day(s) Test Set Up
Monday-Friday
CPT/LOINC
CPT: 81241
LOINC:
Factor V Leiden Mutation: 91712-0
Factor V Leiden Method Comment: 85069-3
Factor V Leiden Limitations: 77202-0
Reference Values
Normal (No mutation detected)