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HelpTest Code LAB7688 Coagulation Factor II Activity Assay, Plasma
Additional Codes
| Test Name in EPIC | EPIC Test Code | Mnemonic |
|---|---|---|
| FACTOR 2 ASSAY | LAB7688 | FAC2 |
Useful For/Utility
Diagnosing congenital deficiency (rare), evaluating acquired deficiencies associated with liver disease or vitamin K deficiency, oral anticoagulant therapy, and antibody-induced deficiencies (eg, in association with lupus-like anticoagulant), determining warfarin treatment stabilization in patients with nonspecific inhibitors (ie, lupus anticoagulant), determining degree of anticoagulation with warfarin to correlate with level of protein S, or investigation of prolonged prothrombin time or activated partial thromboplastin time.
Methodology
Electromechanical Clot Detection
Clinical Information
Factor II (prothrombin) is a vitamin K-dependent serine protease synthesized in liver. It participates in the final common pathway of coagulation, as the substrate for the prothrombinase enzyme complex. Prothrombin is the precursor of thrombin (IIa), which converts fibrinogen to fibrin. Plasma biological half-life is about 3 days. Deficiency of factor II may cause prolonged prothrombin time and activated partial thromboplastin time. Deficiency may result in a bleeding diathesis.
Specimen Requirements
Specimen Type: Platelet-poor plasma
Container/Tube:
Fill Blue top tube to the etched line located just below the cap of the tube.
Preferred: Full Light-blue top (3.2% sodium citrate)
Acceptable: Full Sodium Citrate (BD® or Greiner tubes
Specimen Volume: 1 mL
Specimen Minimum Volume: 0.5 mL
Collection Instructions:
1. Do not spin in refrigerated centrifuge.
2. Unacceptable specimens: Specimens that are short-draws, over-draws, clotted or hemolyzed may yield incorrect results.
3. If sending from a site spin down, remove top 2/3 of plasma, and spin plasma again in a polypropylene (cloudy plastic) vial.
4. Aliquot top 3/4 and place platelet-poor plasma in a polypropylene (cloudy plastic) vial.
5. Glass vials are not acceptable for processing/transport/storage.
Additional Information:
1. Double-centrifuged specimens are critical for accurate results as platelet contamination may cause spurious results.
2. If there is a delay in transport of > 4 hours, send specimen frozen.
Specimen Transport Temperature
Refrigerate* 8 hours/Frozen 15 days at -20° C or 1 month at -80° C OK/Ambient 4 hours at 20° ± 5° C OK
*Note: If the same plasma is also to be used for testing of Factor VII, do not store at 2-8° C.
Day(s) Test Set Up
Upon request (day shift only)
Performing Laboratory
Coagulation-
SMDC Clinical Lab
Test Classification and CPT Coding
85210
Reference Values
| TEST | AGE | % Activity |
|---|---|---|
|
Factor II Activity |
0 – 2 days |
41 - 69 |
|
|
3 days – 30 days |
50 - 73 |
|
|
31 days – 1 Year |
62 - 103 |
|
|
1 Year - 5 Years |
70 - 109 |
|
|
6 Years - 10 Years |
67 - 110 |
|
|
11 Years - 17 Years |
61 - 107 |
|
|
18 Years - 150 Years |
80 - 136 |
Normal, full term newborn infants or healthy premature infant may have decreased levels (greater than or equal to 25%) which may remain below adult levels for greater than or equal to 180 days postnatal. (per Mayo Medical Laboratories;4/2014)