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HelpTest Code LAB7688 Coagulation Factor II Activity Assay, Plasma
Additional Codes
| Test Name in EPIC | EPIC Test Code | Mnemonic |
|---|---|---|
| FACTOR 2 ASSAY | LAB7688 | FAC2 |
Useful For/Utility
Diagnosing congenital deficiency (rare), evaluating acquired deficiencies associated with liver disease or vitamin K deficiency, oral anticoagulant therapy, and antibody-induced deficiencies (eg, in association with lupus-like anticoagulant), determining warfarin treatment stabilization in patients with nonspecific inhibitors (ie, lupus anticoagulant), determining degree of anticoagulation with warfarin to correlate with level of protein S, or investigation of prolonged prothrombin time or activated partial thromboplastin time.
Methodology
Electromechanical Clot Detection
Clinical Information
Factor II (prothrombin) is a vitamin K-dependent serine protease synthesized in liver. It participates in the final common pathway of coagulation, as the substrate for the prothrombinase enzyme complex. Prothrombin is the precursor of thrombin (IIa), which converts fibrinogen to fibrin. Plasma biological half-life is about 3 days. Deficiency of factor II may cause prolonged prothrombin time and activated partial thromboplastin time. Deficiency may result in a bleeding diathesis.
Specimen Requirements
Fill Blue top tube to the etched line located just below the cap of the tube.
1. Light-blue top (3.2% sodium citrate); tube must be properly filled
2. Short-draws, over-draws, clotted or hemolyzed specimens may yield incorrect results.
Specimen Transport Temperature
1. Do not spin in refrigerated centrifuge.
2. Double-centrifuged specimens are critical for accurate results - spin initial specimen, remove top 2/3 of plasma, and spin plasma again in polypropylene vial.*
3. Aliquot top 3/4 of respun specimen and place platelet-poor plasma in polypropylene vial.*
4. If there is a delay in transport or testing for > 4 hours, send specimen frozen.
5. Glass vials are not acceptable for processing, transport or storage.
6. Preferred specimen volume: 1 mL plasma
7. Minimum specimen volume: 0.5 mL plasma
* 2 Aliquot labels will auto print at non-performing sites to double spin
Day(s) Test Set Up
Upon request (day shift only)
Performing Laboratory
Coagulation-
SMDC Clinical Lab
Test Classification and CPT Coding
85210
Reference Values
| TEST | AGE | % Activity |
|---|---|---|
|
Factor II Activity |
0 – 2 days |
41 - 69 |
|
|
3 days – 30 days |
50 - 73 |
|
|
31 days – 1 Year |
62 - 103 |
|
|
1 Year - 5 Years |
70 - 109 |
|
|
6 Years - 10 Years |
67 - 110 |
|
|
11 Years - 17 Years |
61 - 107 |
|
|
18 Years - 150 Years |
80 - 136 |
Normal, full term newborn infants or healthy premature infant may have decreased levels (greater than or equal to 25%) which may remain below adult levels for greater than or equal to 180 days postnatal. (per Mayo Medical Laboratories;4/2014)