Test Code LAB8018 Direct Antiglobulin Test
Additional Codes
Test Name in EPIC | EPIC Test Code | Mnemonic |
---|---|---|
DIRECT COOMBS | LAB8018 | DAT |
Test Alias
DAT, Direct, Direct Coombs, Coombs
Useful For/Utility
The direct antiglobulin test (DAT) is used primarily for the investigation of hemolytic transfusion reactions, hemolytic disease of the fetus and newborn, autoimmune hemolytic anemia, and drug-induced immune hemolysis.
Methodology
Tube Testing
Test Algorithm
Extended DATs using IgG and C-3 specific antisera may be performed at additional charge if Coombs, Direct is positive
An RBC elution and any necessary antibody ID may be performed at additional charge if indicated.
Clinical Information
The direct antiglobulin test (DAT) can determine if red cells have been coated in vivo with immunoglobulin, complement, or both.
Specimen Requirements
Specimen must arrive within 48 hours of draw (<24 hours preferred).
Specimen Type: Whole blood
Container/Tube: Pink top (K2EDTA)-Lavender top (EDTA) is also acceptable.
Specimen Volume: 4-6 mL
Specimen Minimum Volume: 1 mL
Collection Instructions: Send specimen in original tube.
Specimen Transport Temperature
Ambient/Refrigerate OK/Frozen NO
Day(s) Test Set Up
Upon request
Performing Laboratory
Essentia Health Laboratory-Transfusion Services
St. Joseph's Medical Center Lab, SMDC Clinical Lab, Fargo Hosital Lab, St. Mary's - Detroit Lakes Lab
Test Classification and CPT Coding
86880