Test Code LABAHPV Human Papillomavirus (HPV), High Risk with 16 and 18 Genotype by PCR, SurePath
Additional Codes
Test Name in EPIC | EPIC Test Code | Mnemonic |
---|---|---|
ANAL HPV ONLY BY PCR | LABAHPV | SP HPV1618 |
Methodology
Qualitative Polymerase Chain Reaction
Specimen Requirements
Specimen Preparation
Transport original SurePath or briefly vortex and transfer 3 mL to an ARUP Standard Transport Tube. (Min 1.5 mL)
Storage/Transport Temperature
Refrigerated.
Unacceptable Conditions
Bloody or dark brown specimens. Specimens in any media other than indicated above.
Specimen source is required.
Specimen Type: Monolayer anal pap smear collection
Container/Tube: SurePath liquid based pap test media collection vial
Specimen Volume: Dacron/Polyester swab in liquid media (SurePath vial)
Collection Instructions:
1. Obtain an adequate sampling of anal transformation zone by inserting a water moistened Dacron/Polyester swab into the anal canal 5-6 cm.
2. Using gentle pressure, rotate the swab to sample all sides of the anal canal.
3. After collecting the specimen, place the swab directly into the SurePath vial, vigorously swirling the swab while pressing against the wall of the vial. You may break or cut the tip off of the collection device and leave it in the SurePath vial. For a more cellular specimen, you may repeat this collection a second time with a new Dacron/Polyester swab and place it into the same SurePath vial.
4. Tighten cap.
5. Label container with patient's full name, Medical Record Number, Date of Birth, provider's name, date and actual time of collection, and specimen type. A patient label containing this information is preferred.
6. Place vial in a plastic specimen bag with request form inserted into side pocket separate from specimen.
7. Enter in Order Comment, "Anal specimen, collected in SurePath Medium."
Additional Information:
1. The following specimens are at risk of being discarded:
a. No requisition form or EPIC order
b. Name on requisition or EPIC order does not match name on specimen
c. Unlabeled specimen
d. No provider name given
Specimen Transport Temperature
Room Temp
Day(s) Test Set Up
Performed Mon, Wed, Fri 1-5 days
Performing Laboratory
ARUP
Test Classification and CPT Coding
CPT 87626
Reference Values
Reference IntervalMore information
Negative
Interpretive DataMore information
This test amplifies DNA of HPV16, HPV18 and 12 other high-risk HPV types (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) associated with cervical cancer and its precursor lesions. Sensitivity may be affected by specimen collection methods, stage of infection, and the presence of interfering substances. Results should be interpreted in conjunction with other available laboratory and clinical data. A negative high-risk HPV result does not exclude the presence of other high-risk HPV types the possibility of future cytologic abnormalities, underlying CIN2-3, or cancer.
HPV testing should not be used for screening or management of atypical squamous cells of undetermined significance (ASCUS) in women under age 21.