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HelpTest Code LABCD20Q CD20 B Cell Quantitation by Flow Cytometry, Peripheral Blood
Additional Codes
| Test Name in EPIC | EPIC Test Code | Mnemonic |
|---|---|---|
| CD20 B CELL QUANTITATION BY FLOW CYTOMETRY | LABCD20Q | CD20Q |
Useful For/Utility
For enumeration of B cell lymphocytes. Includes percent cells and absolute counts for CD19 and CD20.
This assay should not be used for diagnosing lymphocytic malignancies or evaluation of lymphocytosis of unknown etiology.
To evaluate an individual's major lymphocyte subsets - T, B and Natural Killer (NK) cells, order T & B Cell Markers (LAB2705).
Methodology
Immunophenotyping by Flow Cytometry
Purpose: CD19+ and CD20+ lymphocyte percentage and absolute counts may be useful for evaluation of B Cells in immunodeficiency states or therapeutic B Cell depletion.
Includes percent cells and absolute counts for CD19 (B Cells) and CD20 (B Cells).
Clinical Information
CD19+ and CD20+ lymphocyte percentage and absolute counts may be useful for evaluation of B Cells in immunodeficiency states or therapeutic B Cell depletion.
Specimen Requirements
Specimen Type: Peripheral whole blood
Container/Tube: 4 mL lavender top EDTA
Specimen Volume: Full tube
Specimen Minimum Volume: 1 mL
Collection Instructions:
1.Send specimen in original tube. Do not aliquot.
2.Forward promptly to Flow Cytometry Lab at ambient temperature.
3.It is recommended that specimens arrive within 24 hours of draw. Draw and package specimen as close to shipping time as possible.
4.Testing must be completed within 48 hours of specimen collection.
Specimen Transport Temperature
Ambient Preferred/Refrigerate NO/Frozen NO
Day(s) Test Set Up
Monday through Sunday (day shift only)
Performing Laboratory
SMDC Clinical Laboratory - Special Hematology
Test Classification and CPT Coding
86355 - Absolute B Cell Count
Reference Values
| % | Absolute Count (Cells/ul | |
| CD19+ | 3.2 - 21.9 | 36 - 450 |
| CD20+ | 3.2 - 21.9 | 36 - 450 |