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Test Code LABENTPA Enteric Pathogens, Stool

Important Note

This test is not appropriate for inpatients who have been hospitalized for more than 3 days and any specimen submitted on such patients will be rejected.

Clostridium difficile LABCDIFP-Clostridium difficile by Molecular Detection

For Central Region Only:

Salmonella and Shigella will reflex to sensitivity testing in Microbiology, only when detected. The Cary Blair vial will be plated to BAP, EMB, and Hektoen for isolation to perform the sensitivity.

Infectious

Additional Codes

Test Name in EPIC EPIC Test Code  Mnemonic Order LOINC
ENTERIC PATHOGENS BY NUCLEIC ACID TESTING LABENTPA Enteric Path 82195-9

 

Methodology

Nucleic Acid Multiplexed Detection

Includes the following enteric pathogens: Campylobacter, Norovirus GI/GII, Rotavirus A, Salmonella, Shiga-like toxin-producing Escherichia coli (STEC) stx1/stx2 (including specific identification of the E. coli 0157 serogroup with STEC), Shigella, Vibrio, and Yersinia enterocolitica, Plesiomonas shigelloides, Enteroaggregative Escherichia coli, Enteropathogenic Escherichia coli, Enterotoxigenic Escherichia coli, Sheigella/Enteroinvasive Escherichia coli (EIEC), Cryptosporidium, Cyclospora cayetanensis, Entamoeba histolytica, Giardia lamblia (also known as G. intestinalis and G. duodenalis), Adenovirus F40/41, Astrovirus, and Sapovirus (I, II, IV, V)

Specimen Requirements

Whenever possible, specimens should be obtained before antibiotics or antimicrobial agents have been administered.

 

Submit only 1 of the following specimens:

 

Unpreserved specimen must arrive at the testing laboratory within 2 hours of collection at ambient (room) temperature.

 

Specimen Type: Human stool, unpreserved

Container/Tube: Sterile container, Clean Container acceptable

Specimen Volume: 1 g of stool (or approximately 1 Tablespoon)

Collection Instructions:

1. Collect stool in sterile container or transfer from bedpan or plastic wrap stretched over toilet bowl.

2. Label container with patient’s name (first and last), date and actual time of collection, and type of specimen.
Additional Information:

1. Stool contaminated with urine or submitted in diaper is not acceptable.

2. Patient should avoid recent antibiotics or x-ray contrast material, antidiarrheal medications, antacids, bismuth, castor oil instillation, or Metamucil.

3. Multiple specimens from the same day will be rejected. Specimens must be collected at least 24 hours apart.

 

 

Preserved, refrigerated specimen must arrive at the testing lab within 48 hours of collection.

 

Specimen Type: Human stool, preserved

Container/Tube: 1 Orange top stool transport sterile vial containing Carey-Blair based media

Specimen Volume: 1 g of stool per vial (or approximately 1 Tablespoon)

Collection Instructions:

1. Collect stool in a sterile container or transfer from bedpan or plastic wrap stretched over toilet bowl.

2. Within 2 hours of patient passing stool, place specimen into orange top preservative vial. Portions of stool which contain pus, blood, or mucous should be sampled. Add stool until liquid reaches red line. Do not fill above line indicated on container.

3. Do not overfill.

4. Cap vial tightly and mix well.

5. Label vial with patient’s name (first and last), date, actual time of collection, and type of specimen.

Additional Information:

1. Stool contaminated with urine or submitted in diaper is not acceptable.

2. Patient should avoid recent antibiotics or x-ray contrast material, antidiarrheal medications, antacids, bismuth, castor oil instillation, or Metamucil.

3. Multiple specimens from the same day will be rejected. Specimens must be collected at least 24 hours apart.

 

Specimen Transport Temperature

Unpreserved Stool-Ambient 2 hours OK/Refrigerate NO//Frozen NO

Preserved Stool- Ambient 4 days (Preferred)/Refrigerate 4 days OK//Frozen NO

 

Day(s) Test Set Up

Monday through Sunday, day shift only

Performing Laboratory

Microbiology -

Central Region: St. Joseph's Medical Center Lab

East Region: SMDC Clinical Lab

West Region: Fargo Hospital Lab

 

Test Classification and CPT Coding

87507

Additional CPT codes will be billed when appropriate.

Rejection Criteria

Multiple specimens from the same day will be rejected. Specimens must be collected at least 24 hours apart.

Report Available

24-48 hours

Cautions

This test is acceptable for pediatric patients.

Rare Norovirus and Rotavirus strains may not be detected due to high-genetic diversity in some variants.

Campylobacter pathogenic species (coli, jejuni, and lari) are included in this panel, however they will not be individually identified. Also, non-pathogenic Campylobacter insulaenigrae may yield a positive result in rare instances..

Reference Values

Negative

Special Instructions