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Test Code LABHEPXA Heparin Anti-Xa Assay

Additional Codes

Test Name in EPIC EPIC Test Code Mnemonic
HEPARIN ANTI-XA ASSAY LABHEPXA HEPXA

 

Methodology

Chromogenic  Assay

Specimen Requirements

Specimens must be centrifuged and plasma separated from cells within 1 hour of collection.

 

Specimen Type: Platelet-poor plasma 

Container/Tube:

Fill Blue top tube to the etched line located just below the cap of the tube.

Preferred: Full Light-blue top (3.2% sodium citrate)

Acceptable:  Full Sodium citrate (BD® or Greiner tubes)

Specimen Volume: 1 mL

Specimen Minimum Volume: 0.5 mL

Collection Instructions:

1. Draw level 6 hr after start of infusion and then 6 hours after each change.

2. Unacceptable specimens: Specimens that are short-draws, over-draws, clotted or hemolyzed may yield incorrect results.

3. If sending from a site spin down, remove top 2/3 of plasma, and spin plasma again in a polyproyplene (cloudy plastic) vial.

4. Aliquot top 3/4 and place platelet-poor plasma in a polypropylene (cloudy plastic) vial.

5. Glass vials are not acceptable for processing/transport/storage.

Additional Information:

1. Double-centrifuged specimens are critical for accurate results as platelet contamination may cause spurious results.

2. If there is a delay in transport of >2 hours, send specimen frozen.

3. To monitor Enoxaparin (Lovenox) therapy, draw for peak 4-6 hours post dose.

Specimen Transport Temperature

Frozen OK/Ambient 2 hours OK/Refrigerate NO

Day(s) Test Set Up

Monday through Sunday (all shifts)

Performing Laboratory

Coagulation-

Deer River Hospital Lab, Northern Pines Lab, Sandstone Hospital Lab, SMDC Clinical Lab, St. Joseph's Medical Center Lab, St. Mary's Hospital - Superior Lab, Virginia Hospital Lab

Ada Hospital Lab, Fargo Hospital Lab, Fosston Hospital Lab, Holy Trinity Hospital Lab, St. Mary's - Detroit Lakes Hospital Lab

Test Classification and CPT Coding

85520

Reference Values

Therapeutic Ranges: 

 

Range: IU/mL

 

Comments: This test is not validated for other direct Xa inhibitor drugs (e.g. Rivaroxaban, Apixaban, etc.)

 

*Unfractionated Heparin:

Therapeutic range may vary from 0.2 to 0.7 IU/mL. The upper and lower limit of therapeutic range varies based on intensity (indication), see dosing nomogram.

 

*Low Molecular Weight Heparin:

Enoxaprin (Lovenox):  0.6 to 1.0 IU/mL (for 1mg/kg twice daily dosing), 1 to 2 IU/mL (for 1.5 mg/kg once daily dosing)

**Other LMWH products may yield different therapeutic ranges. Please consult drug product insert for appropriate range.