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Test Code LABHHV6AB Human Herpesvirus 6 (HHV-6A and HHV-6B) by Quantitative PCR

Additional Codes

Test Name in EPIC EPIC Test Code Mnemonic ARUP Test ID
Human Herpesvirus 6(HHV-6A,HHV-6B) Quant PCR LABHHV6AB HHV6PCR 0060071

 

Useful For/Utility

Detect and quantify HHV6 subtypes A and B in immunocompromised patients.

Methodology

Quantitative Polymerase Chain Reaction

Specimen Requirements

Collect    
Lavender (EDTA), pink (K2EDTA), serum separator tube, or CSF.

Specimen Preparation    
Separate serum or plasma from cells. Transfer 1 mL serum, plasma or CSF to a sterile container. (Min: 0.5 mL)

Unacceptable Conditions    
Heparinized specimens, tissues in optimal cutting temperature compound.

Remarks    
Specimen source required.

Specimen Transport Temperature

Storage/Transport Temperature    
Frozen.

Test Classification and CPT Coding

87533

LOINC

    HHV6 by PCR Source    31208-2
    HHV6 by PCR Type    38348-9
    HHV6 Quant by PCR (copy/mL)    38349-7
    HHV6 Quant by PCR (log copy/mL)    38350-5
    HHV6 by PCR Interp    51730-0
    HHV6 Quant by PCR (log IU/mL)    100680-8
    HHV6 Quant by PCR (IU/mL)    101255-8

Day(s) Performed

Tues-Sat

Reported 1-4 days

Reference Values

Not detected

Interpretation

The quantitative range of this assay is 2.7-6.7 log copies/mL (500-5,000,000 copies/mL) or 3.1-7.1 log IU/mL (1250-12,500,000 IU/mL).

1 copies/mL is approximately 2.5 IU/mL.

A negative result (less than 2.7 log copies/mL or less than 500 copies/mL; less than 3.1 log IU/mL or less than 1250 IU/mL) does not rule out the presence of PCR inhibitors in the patient specimen or HHV6 DNA in concentrations below the level of detection of the assay. Inhibition may also lead to underestimation of viral quantitation.

The limit of quantification for this DNA assay is 2.7 log copies/mL (500 copies/mL) or 3.1 log IU/mL (1250 IU/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<2.7 log copies/mL (<500 copies/mL). and <3.1 log IU/mL (<1250 IU/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."

This assay detects and quantifies HHV6 subtypes A and B.