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HelpTest Code LABHLA2T Human Leukocyte Antigens (HLA)-DR-DQ Disease Association Typing Low Resolution, Blood
Additional Codes
| Test Name in EPIC | EPIC Test Code | Mnemonic | Mayo Test ID |
|---|---|---|---|
| HLA Class II Molecular Typing Disease Association | LABHLA2T | 2DIS | 2DIS |
Specimen Required
Container/Tube: Yellow top (ACD solution A or B)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood in original tube. Do not aliquot.
Additional Information: Specimen acceptability is based on extracted DNA concentration and not sample age.
Useful For
Determining class II human leukocyte antigens (HLA) to identify potential disease associations or markers for drug hypersensitivity
Method Name
Polymerase Chain Reaction (PCR)/Next-Generation Sequencing (NGS)
Reporting Name
HLA-DR-DQ DisAssoc Typing LowRes,BSpecimen Type
Whole Blood ACDSpecimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Extracted DNA | Reject |
Clinical Information
Human leukocyte antigen (HLA) class II genes (HLA-DRB1, -DRB3/4/5, -DQA1, -DQB1, -DPA1, -DPB1) are a part of the major histocompatibility gene complex that encodes for proteins involved in immune recognition.
Reference Values
Not applicable
Interpretation
Interpretation depends on the rationale for ordering the test.
Cautions
No significant cautionary statements.
Method Description
Next-generation sequencing is used to type for class II alleles (DRB1, DRB3/4/5, DQB1, DQA1, DPB1, and DPA1) from genomic DNA. This method uses strictly controlled polymerase chain reaction (PCR) conditions for DNA amplification. The PCR amplicons are processed and sequenced via the Illumina MiSeq instrument. The output files are analyzed in provided software, which compares the data against the IMGT/HLA database to assign the molecular typing.(Package insert: NGSgo HLA Kit. GenDx; v3, 06/2022)
For resolution of an allelic ambiguity or in select cases, the following additional methodologies may be utilized:
-Sequence-based typing (SBT) by Sanger sequencing(Package insert: SeCore Sequencing and GSSP Kits. One Lambda, Inc; Rev 3, 02/06/2021)
-Reverse sequence-specific oligonucleotides (SSO)(Package insert: LABType SSO Typing Test. One Lambda, Inc.; Rev 04, 11/11/2019)
Day(s) Performed
Monday, Wednesday
Report Available
7 to 17 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81375
81376 x3
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| 2DIS | HLA-DR-DQ DisAssoc Typing LowRes,B | 96640-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 2DA02 | DRDQ DisAssoc Comment | 96625-9 |
| 2DA03 | DRB1 - 1 Equivalent | 57298-2 |
| 2DA04 | DRB1 - 2 Equivalent | 57298-2 |
| 2DA05 | DRB1 - 1 Molecular | 96664-8 |
| 2DA06 | DRB1 - 2 Molecular | 96664-8 |
| 2DA07 | DRB345 - 1 Equivalent | 96673-9 |
| 2DA08 | DRB345 - 2 Equivalent | 96673-9 |
| 2DA09 | DRB345 - 1 Molecular | 96672-1 |
| 2DA10 | DRB345 - 2 Molecular | 96672-1 |
| 2DA11 | DQB1 - 1 Equivalent | 53938-7 |
| 2DA12 | DQB1 - 2 Equivalent | 53938-7 |
| 2DA13 | DQB1 - 1 Molecular | 78017-1 |
| 2DA14 | DQB1 - 2 Molecular | 78017-1 |
| 2DA15 | DQA1 - 1 Molecular | 96654-9 |
| 2DA16 | DQA1 - 2 Molecular | 96654-9 |
| 2DA17 | DPB1 - 1 Molecular | 96648-1 |
| 2DA18 | DPB1 - 2 Molecular | 96648-1 |
| 2DA19 | DPA1 - 1 Molecular | 96643-2 |
| 2DA20 | DPA1 - 2 Molecular | 96643-2 |
| LRTM2 | Test Method | 85069-3 |
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.