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HelpTest Code NECT4 Nectin-4, Semi-Quantitative Immunohistochemistry, Manual
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.
Necessary Information
A pathology/diagnostic report and a brief history are required.
Specimen Required
Specimen Type: Tissue
Supplies: Pathology Packaging Kit (T554)
Collection Instructions: Formalin-fixed, paraffin-embedded tissue block; or 3 unstained glass, positively-charged slides with 4-microns formalin-fixed, paraffin-embedded tissue
Additional Information: One slide will be stained with hematoxylin and eosin and returned.
Useful For
Identifying nectin-4 overexpression in bladder cancer and other cancers
Method Name
Immunohistochemistry (IHC)
Reporting Name
Nectin-4, SemiQuant IHC, ManualSpecimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Special | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Wet/frozen tissue Decalcified paraffin embedded tissue Cytology smears Non-formalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides Snowcoat slides |
Reject |
Clinical Information
Nectin-4, also known as poliovirus receptor-like 4 (PVRL4), is overexpressed in many cancers, in particular bladder cancer. An antibody drug conjugate, enfortumab vedotin, targets the cell surface protein nectin-4. Some studies suggest that tumors with high expression of nectin-4 are more likely responsive to drug therapy.
Reference Values
An interpretive report will be provided.
Interpretation
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Cautions
This test has been validated for nondecalcified paraffin-embedded tissue specimens fixed in 10% neutral buffered formalin. Recommended fixation time is between 6 and 48 hours. This test has not been validated on tissues subjected to the decalcification process or use of alternative fixatives.
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks.
Method Description
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Report Available
5 to 7 daysSpecimen Retention Time
Until reportedPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88360
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| NECT4 | Nectin-4, SemiQuant IHC, Manual | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 622261 | Interpretation | 59465-5 |
| 622262 | Participated in the Interpretation | No LOINC Needed |
| 622263 | Report electronically signed by | 19139-5 |
| 622264 | Material Received | 81178-6 |
| 622265 | Disclaimer | 62364-5 |
| 622266 | Case Number | 80398-1 |